| identifier | PRJEB2672 | ||||||||||||
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| type | bioproject | ||||||||||||
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| title | Combining SNP discovery from next-generation sequencing data with Bulked Segregant Analysis to fine-map genes in polyploid wheat | ||||||||||||
| description | Next generation sequencing (NGS) technologies are providing new ways to accelerate fine-mapping and gene isolation in many species. To date, the majority of these efforts have focused on diploid organisms with readily available whole genome sequence information. In this study, as a proof of concept, we tested the use of NGS for SNP discovery in tetraploid wheat lines differing for the previously cloned grain protein content (GPC) gene GPC-B1. Bulked segregant analysis (BSA) was used to define a subset of putative SNPs within the candidate gene region, which were then used to fine-map GPC-B1. | ||||||||||||
| data type | Transcriptome or Gene expression | ||||||||||||
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| distribution | JSONJSON-LD | ||||||||||||
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| status | public | ||||||||||||
| visibility | unrestricted-access | ||||||||||||
| dateCreated | 2012-01-11T00:00:00Z | ||||||||||||
| dateModified | 2012-01-11T00:00:00Z | ||||||||||||
| datePublished | 2012-01-11T00:00:00Z |