home > bioproject > PRJEB2786
identifier PRJEB2786
type bioproject
title A modified method for whole exome resequencing from minimal amounts of starting DNA
description "Next generation DNA sequencing (NGS) technologies have revolutionized the pace at which whole genome and exome sequences can be generated. However, despite these advances, many of the methods for targeted resequencing, such as the generation of high-depth exome sequences, are somewhat limited by the relatively large amounts of starting DNA that are normally required. In the case of tumour analysis this is particularly pertinent as many tumour biopsies often return submicrogram quantities of DNA, especially when tumours are microdissected prior to analysis. Here, we present a method for exome capture and resequencing using as little as 50 ng of starting DNA. The sequencing libraries generated by this minimal starting amount (MSA-Cap) method generate datasets that are comparable to standard amount (SA) whole exome libraries which use three micrograms of starting DNA. This method, which can be performed in most laboratories using commonly available reagents, has the potential to enhance large scale profiling efforts such as the resequencing of tumour exomes."
data type Exome
The Breakthrough Toby Robins Breast Cancer Research Centre, The Institute of Cancer Research, United Kingdom
sra-run  ERR058814ERR058815ERR058816ERR058817ERR058818ERR058819ERR058820ERR058821
sra-submission  ERA073220
biosample  SAMEA1094647SAMEA1094650SAMEA1094648SAMEA1094649
sra-sample  ERS077570ERS077571ERS077573ERS077572
sra-experiment  ERX036660ERX036661ERX036662ERX036663ERX036664ERX036665ERX036666ERX036667
distribution JSONJSON-LD
status public
visibility unrestricted-access
dateCreated 2012-02-08T00:00:00+0000
dateModified 2012-02-08T00:00:00+0000
datePublished 2012-02-08T00:00:00+0000