| identifier | PRJEB37669 | ||
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| type | bioproject | ||
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| title | Haplotype resolved chromosome level assembly of Apricot generated by application of gamete binning on single cell sequencing data of gametes. | ||
| description | We developed and applied a novel gamete binning strategy on single cell sequencing data of gametes to generate haplotype resolved chromosome level assembly of Apricot genome. This study includes the data created in the work of "Chromosome-level and haplotype-resolved genome assembly enabled by high-throughput single-cell sequencing of gamete genomes”, where a novel method has been developed to achieve the haploid assemblies in heterozygous species. As an application, two haploid genomes were assembled for an apricot tree (cultivar Rojo Pasion;isolate pruArmRojPasFocal), using PacBio reads sequenced from the somatic tissues and 10x Genomics/Illumina short reads sequenced from the hundreds of gamete genomes.Specifically, here are the assembly and annotation of the two haploid genomes from a single diploid heterozygous individual (isolate pruArmRojPasFocal), referred to as "Currot" and “Orange Red” haplotypes. Following are the details of the assemblies.
The project also contains raw read data for
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| data type | Genome sequencing and assembly | ||
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| distribution | JSONJSON-LD | ||
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| status | public | ||
| visibility | unrestricted-access | ||
| dateCreated | 2020-04-12T00:00:00Z | ||
| dateModified | 2020-04-12T00:00:00Z | ||
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