home > bioproject > PRJEB9179
identifier PRJEB9179
type bioproject
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organism
title TSAR1 and TSAR2 activate triterpene saponin production in Medicago truncatula
description We identified two novel transcription factors ‘Triterpene Saponin Activation Regulator’ (TSAR) 1 and 2 that each activate a specific branch of saponin synthesis in M. truncatula. Therefore we generated three independent TSAR1 and three independent TSAR2 overexpression (OE) hairy root lines. As a control we raised three independent hairy root lines expressing a non-functional GUS gene. By qRT-PCR analyses and metabolite profiling of these lines, we found that TSAR1 OE leads to accumulation of non-haemolytic saponins whereas TSAR2 instigates accumulation of haemolytic saponins. To identify additional targets of TSAR1 and TSAR2 we carried out a genome-wide transcript profiling study by RNA-Seq.
data type Transcriptome or Gene expression
organization
publication
properties 
{...}
dbXrefs
sra-run  ERR865492ERR865493ERR865494ERR865495ERR865496ERR865497ERR865498ERR865499ERR865500
sra-submission  ERA433848
biosample  SAMEA3359857SAMEA3359861SAMEA3359864SAMEA3359863SAMEA3359858SAMEA3359856SAMEA3359859SAMEA3359862SAMEA3359860
sra-study  ERP010262
sra-sample  ERS713353ERS713357ERS713360ERS713359ERS713354ERS713352ERS713355ERS713358ERS713356
sra-experiment  ERX945121ERX945125ERX945128ERX945127ERX945122ERX945120ERX945123ERX945126ERX945124
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status public
visibility unrestricted-access
dateCreated 2015-12-01T00:00:00Z
dateModified 2015-12-01T00:00:00Z
datePublished 2015-11-30T00:00:00Z