identifier |
PRJEB9179 |
type |
bioproject |
sameAs |
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organism |
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title |
TSAR1 and TSAR2 activate triterpene saponin production in Medicago truncatula |
description |
We identified two novel transcription factors ‘Triterpene Saponin Activation Regulator’ (TSAR) 1 and 2 that each activate a specific branch of saponin synthesis in M. truncatula. Therefore we generated three independent TSAR1 and three independent TSAR2 overexpression (OE) hairy root lines. As a control we raised three independent hairy root lines expressing a non-functional GUS gene. By qRT-PCR analyses and metabolite profiling of these lines, we found that TSAR1 OE leads to accumulation of non-haemolytic saponins whereas TSAR2 instigates accumulation of haemolytic saponins. To identify additional targets of TSAR1 and TSAR2 we carried out a genome-wide transcript profiling study by RNA-Seq. |
data type |
Transcriptome or Gene expression
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organization |
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publication |
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dbXrefs |
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distribution |
JSONJSON-LD
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status |
public |
visibility |
unrestricted-access |
dateCreated |
2015-12-01T00:00:00Z |
dateModified |
2015-12-01T00:00:00Z |
datePublished |
2015-11-30T00:00:00Z |