| description |
16S sequencing was used to study the adaptation of a soil microbiome to the addition of a focal species, Pseudomonas fluorescens that was either preadapted or not to the soil environment.The results indicate that community structure is shaped by evolutionary priority effects.N501f and N701r primers used on the V4 regionForward and reverse amplification primers: 5'-ACACTCTTTCCCTACACGACGCTCTTCCGATCTNNNNNGTGCCAGCMGCCGCGGTAA-3'5'-GTGACTGGAGTTCAGACGTGTGCTCTTCCGATCTGGACTACHVGGGTWTCTAAT-3'N501f and N701r primers5'-AATGATACGGCGACCACCGAGATCTACACTAGATCGCACACTCTTTCCCTACACGACGCTC-3'5'-CAAGCAGAAGACGGCATACGAGATTCGCCTTAGTGACTGGAGTTCAGACGTGTGCTC-3' |