| identifier | PRJNA150133 | ||
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| type | bioproject | ||
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| organism | Mus musculus | ||
| title | Transcriptome of double-positive thymocyte precursors transduced with a retrovirus encoding Lin28a | ||
| description | We performed paired-end RNA-seq to compare the transcriptome of DP thymocytes that ectopically express Lin28 in vivo versus untransduced (GFP-ve) DP thymocytes.Overall design: Transduced (GFP+) and untransduced (GFP-) CD4+ CD8+ CD3- thymocytes were sorted and pooled from three recipients of hematopoietic stem and progenitor cells transduced with Lin28-RV six weeks post-reconstitution. Total RNA was extracted and paired-end library construction and sequencing was performed on oligo-dT purified RNA. | ||
| data type | Transcriptome or Gene expression | ||
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