identifier | PRJNA171220 | ||
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type | bioproject | ||
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organism | Saccharomyces cerevisiae | ||
title | Genome wide profiling of RNA polymerase III in Saccharomyces cerevisiae | ||
description | Genome wide mapping of RNA polymearase III binding sites in Saccharomyces cerevisiae under normal growth and nutrient starved condition using ChIP-seq. Chromatin Immuno-precipitation (ChIP) was performed for FLAG tagged version of pol III subunit RPC128 after crosslinking the log-phase cells with formaldehyde. MOCK and IP DNA was sequenced and coverage of pol III was calculated at each base of the genome.Overall design: RPC128-FLAG ChIP-seq single end seqquencing on Illumina GAII. 2 replicates of IP samples and 1 MOCK sample. Done in under normal growth and nutrient deprivation (4 hours). | ||
data type | Epigenomics | ||
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