| description |
It is a model organism for studying the role of miRNAs in stem cell function. Next generation sequencing technology was used to identify the miRNAs of D. japonica. Bioinformatic analysis showed that 262 miRNA and miRNA* sequences were discovered, of which 102 miRNAs were the same as Schmidtea mediterranea (S. mediterranea) and 160 miRNAs were related to other animals. To identify the functional small RNAs during planarian regeneration, we collected 5 sample, which were whole body, head part, tail part, left part and right part of planarians. Each sample needed about 50 planarians and they were collected from a mountain creek in Zibo, Shandong Province. |