| description |
Cassava Mosaic Disease (CMD) is one of the main biotic and economically important constraints ofcassava (Manihot esculenta Crantz) cultivation in southern Africa. South African cassava mosaic virus(SACMV-[Za:99]) in particular is endemic to South Africa. Compatible virus infection induces andsuppresses host gene expression at the global level. These gene-expression changes are themolecular basis of symptom development and general stress and defence-like responses of the host.Currently, only limited amounts of expression data are available for geminivirus infection in bothmodel and natural host systems. We performed the first next-generation sequencing (NGS) study usingthe ABI SOLiD platform to identify gene expression changes associated with disease developmentand recovery in two cassava (Manihot esculenta) landraces, susceptible T200 and tolerant TME3,induced by SACMV- [ZA:99] infection. Analysis of the SACMV-[ZA:99] infection was carried out at three timepoints (12, 32 and 67 days post inoculation) in T200 and TME3. |