home > bioproject > PRJNA276094
identifier PRJNA276094
type bioproject
sameAs
organism
title Double-digest RAD Sequencing using Ion Proton semiconductor platform (ddRADseq-ion) with non-model organisms
description We have developed a new method for generating single nucleotide polymorphisms (SNPs) using next-generation sequencing. RAD Sequencing has become an established method for SNP genotyping on Illumina sequencing platforms. With a modification to the double-digest RAD Sequencing methodology on Illumina, we have developed a protocol and new adapters for using Ion Torrent semiconductor sequencing. To validate and demonstrate the method, we sequenced 13 genomic libraries of three non-model vertebrate species (two fishes and a lizard).
data type raw sequence reads
organization
publication
properties 
{...}
dbXrefs
sra-run  SRR1814176SRR1814177SRR1814178SRR1814179SRR1814180SRR1814181SRR1814182SRR1814183SRR1814185SRR1814187 More
sra-submission  SRA244008
biosample  SAMN03353729SAMN03353730SAMN03353735SAMN03353740
sra-study  SRP055456
sra-sample  SRS853447SRS853448SRS853449SRS853450
sra-experiment  SRX886552SRX886553SRX886554SRX886555SRX886556SRX886557SRX886558SRX886559SRX886560SRX886561 More
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status public
visibility unrestricted-access
dateCreated 2015-02-23T00:00:00Z
dateModified 2015-02-23T00:00:00Z
datePublished