identifier | PRJNA276094 | ||||||||||||
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type | bioproject | ||||||||||||
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title | Double-digest RAD Sequencing using Ion Proton semiconductor platform (ddRADseq-ion) with non-model organisms | ||||||||||||
description | We have developed a new method for generating single nucleotide polymorphisms (SNPs) using next-generation sequencing. RAD Sequencing has become an established method for SNP genotyping on Illumina sequencing platforms. With a modification to the double-digest RAD Sequencing methodology on Illumina, we have developed a protocol and new adapters for using Ion Torrent semiconductor sequencing. To validate and demonstrate the method, we sequenced 13 genomic libraries of three non-model vertebrate species (two fishes and a lizard). | ||||||||||||
data type | raw sequence reads | ||||||||||||
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distribution | JSONJSON-LD | ||||||||||||
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status | public | ||||||||||||
visibility | unrestricted-access | ||||||||||||
dateCreated | 2015-02-23T00:00:00Z | ||||||||||||
dateModified | 2015-02-23T00:00:00Z | ||||||||||||
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