| description |
First generation animals (F1) were exposed to multiple courses of synthetic glucocorticoids in utero. Animals were then bred to produce second (F2) and third (F3) generation offspring. These animals underwent behavioural assessmenets before sacrifice. In this experiment the prefrontal cortex for F1, F2, and F3 female animals, as well as F1 males (Beta and Control) have been extracted via micro-punch and RNA has been extracted.Overall design: F1 Female Control n=4 vs F1 Female Beta n=4, F2 Female Control n=4 vs F2 Female Beta n=4, F3 Female Control n=4 vs F3 Female Beta n=4, F1 Males Control n=5 vs F1 Males Beta n=6 |