home > biosample > SAMD00000690
identifier SAMD00000690
type biosample
sra-sample  DRS011758
organism Mus musculus musculus
sample_name  DRS011758
sample comment  ES cells carrying a doxycycline-inducible Kdm4b transgene was created using a piggyBac transposon-based expression system from the NCH.4.6 male mouse ES cell line (C57B6/N x C57B6/N) with normal karyotype, and named K4B-ES cells.K4B-ES cells were cultured in the presence or absence of doxycycline, and subjected to fixed chromatin preparation. The fixed chromatin samples (Dminus and Dplus) were used for chromatin immuno-precipitation using anti-histone H3K9me3 polyclonal antibody (Abcam ChIP-grade ab8898). ChIP-Seq libraries were prepared for the immuno-precipitated DNA as well as the input DNA of Dminus and D plus samples using the NEBNext® ChIP-Seq Library Prep Master Mix Set and Multiplex Oligos for Illumina (NEB) according to manufacturer's instructions.
bioproject  PRJDB1790
sra-run  DRR013026
sra-submission  DRA001041
sra-study  DRP001083
sra-sample  DRS011758
sra-experiment  DRX011916
distribution JSONJSON-LD
ddbj_biosample_set.xml.gz  HTTPS FTP
status public
visibility unrestricted-access
dateCreated 2014-05-12T01:17:49+09:00
dateModified 2014-11-17T00:41:36+09:00
datePublished 2014-11-17T00:41:36+09:00