| identifier |
SAMD00000691 |
| type |
biosample |
| sameAs |
|
| organism |
Mus musculus musculus
|
| attributes |
| sample_name |
DRS011759 |
| sample comment |
ES cells carrying a doxycycline-inducible Kdm4b transgene was created using a piggyBac transposon-based expression system from the NCH.4.6 male mouse ES cell line (C57B6/N x C57B6/N) with normal karyotype, and named K4B-ES cells.K4B-ES cells were cultured in the presence or absence of doxycycline, and subjected to fixed chromatin preparation. The fixed chromatin samples (Dminus and Dplus) were used for chromatin immuno-precipitation using anti-histone H3K9me3 polyclonal antibody (Abcam ChIP-grade ab8898). ChIP-Seq libraries were prepared for the immuno-precipitated DNA as well as the input DNA of Dminus and D plus samples using the NEBNext® ChIP-Seq Library Prep Master Mix Set and Multiplex Oligos for Illumina (NEB) according to manufacturer's instructions. |
|
| properties ▽ |
{...}
|
| dbXrefs |
|
| distribution |
JSONJSON-LD
|
| Download |
|
| status |
public |
| visibility |
unrestricted-access |
| dateCreated |
2014-05-12T01:17:49+09:00 |
| dateModified |
2014-11-17T00:41:36+09:00 |
| datePublished |
2014-11-17T00:41:36+09:00 |