home > biosample > SAMD00003524
identifier SAMD00003524
type biosample
sra-sample  DRS011944
organism Mus musculus
sample_name  DRS011944
sample comment  For comprehensive analysis of Ras-dependent changes in H3K27me content, we performed ChIP-seq analyses at various times after Ras induction. We sampled NIH 3T3 cells at 0, 2, 4, 7, and 12 days after infection with the H-Ras(G12V) retroviral vector. ChIP-seq libraries were prepared from ~40 ng each of ChIP and input DNA with the use of a TruSeq DNA LT Sample Prep Kit (Illumina, San Diego, CA). Libraries were clonally amplified in a flow cell and sequenced with the use of HiSeq Control Software 1.5 (Illumina) and a 48-nucleotide paired-end sequence. Image analysis and base calling were performed with the use of Real Time Analysis (RTA) 1.13 software.
bioproject  PRJDB1915
sra-run  DRR013267
sra-submission  DRA001075
sra-study  DRP001122
sra-sample  DRS011944
sra-experiment  DRX012095
distribution JSONJSON-LD
ddbj_biosample_set.xml.gz  HTTPS FTP
status public
visibility unrestricted-access
dateCreated 2014-05-12T01:17:49+09:00
dateModified 2014-11-12T08:28:28+09:00
datePublished 2013-11-27T15:00:00+09:00