| attributes |
| sample_name |
DRS011943 |
| sample comment |
For comprehensive analysis of Ras-dependent changes in H3K27me content, we performed ChIP-seq analyses at various times after Ras induction. We sampled NIH 3T3 cells at 0, 2, 4, 7, and 12 days after infection with the H-Ras(G12V) retroviral vector. ChIP-seq libraries were prepared from ~40 ng each of ChIP and input DNA with the use of a TruSeq DNA LT Sample Prep Kit (Illumina, San Diego, CA). Libraries were clonally amplified in a flow cell and sequenced with the use of HiSeq Control Software 1.5 (Illumina) and a 48-nucleotide paired-end sequence. Image analysis and base calling were performed with the use of Real Time Analysis (RTA) 1.13 software. |
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