| attributes |
| sample_name |
DRS005716 |
| sample comment |
Sorghum (Sorghum bicolor L. Moench) SIL-05, which is resistant to target leaf spot, was used for mRNA seq. Four seeds were sterilized, placed onto Kumiai-Ryujyou-Baido soil (Kureha Chemical, Tokyo, Japan) in each plant box (5×5×15 cm high) and incubated in a chamber for 7 days under 16 h light and 8 h dark at 28°C. On day 7 (about the 2- or 3-leaf stage) the sorghum plants were sprayed with 1 ml of 0.01% Tween-20 solution per plant box. The plants were sampled at 12 h after inoculation. Four biological replicates were collected, immediately frozen in liquid nitrogen, and mixed, to minimize the effect of transcriptome unevenness among plants. Total RNA from 4 plants was extracted with RNeasy (Qiagen, Hilden, Germany). The total RNA was subjected to further analysis. mRNA-seq library construction and sequencing were performed at Hokkaido System Science Co., Ltd. (Sapporo, Japan) in accordance with the manufacturer’s instructions by using an Illumina (San Diego, CA) TruSeq RNA Sample Prep Kit and 100-bp single-end protocol on a HiSeq2000 system (Illumina). |
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