| attributes |
| sample_name |
DRS005717 |
| sample comment |
Sorghum (Sorghum bicolor L. Moench) SIL-05, which is resistant to target leaf spot, was used for mRNA seq. Four seeds were sterilized, placed onto Kumiai-Ryujyou-Baido soil (Kureha Chemical, Tokyo, Japan) in each plant box (5×5×15 cm high) and incubated in a chamber for 7 days under 16 h light and 8 h dark at 28°C. Bipolaris sorghicola isolate BC-24 (MAFF number 511379) was used as the inoculum. The BC-24 strain was grown on vegetable juice (Campbell V8) agar for 10 days in the dark at 25°C and then placed under UV light, where it was kept for 10 days to induce conidia formation. Conidia were harvested in 0.01% Tween-20, and the concentration of suspensions was adjusted to 4×105 conidia/ml. On day 7 (about the 2- or 3-leaf stage) the sorghum plants were sprayed with 1 ml of this suspension per plant box. The plants were sampled at 12 h after inoculation. Four biological replicates were collected, immediately frozen in liquid nitrogen, and mixed, to minimize the effect of transcriptome unevenness among plants. Total RNA from 4 plants was extracted with RNeasy (Qiagen, Hilden, Germany). The total RNA was subjected to further analysis. mRNA-seq library construction and sequencing were performed at Hokkaido System Science Co., Ltd. (Sapporo, Japan) in accordance with the manufacturer’s instructions by using an Illumina (San Diego, CA) TruSeq RNA Sample Prep Kit and 100-bp single-end protocol on a HiSeq2000 system (Illumina). |
|