| attributes |
| sample_name |
DRS000990 |
| sample comment |
JF1 females were mated with B6 males to generate [JF1xB6]F1 mice. Total RNA was extracted using ISOGEN (Nippon gene, Tokyo, Japan) and further purified RNeasy mini Kit and RNase-free DNase (Qiagen, CA, USA) from four [JF1xB6]F1 placentas at E13.5, and pooled. Twelve micrograms of total RNA was used for rRNA depletion (RiboMinus Eukaryote kit for RNA-seq, Invitrogen, CA, USA) and RNA-seq library construction (SOLiD Whole Transcriptome Analysis Kit, Life technologies, CA, USA) according to the manufacturer’s protocol. |
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