home > biosample > SAMD00009100
identifier SAMD00009100
type biosample
sra-sample  DRS000989
organism Mus musculus
sample_name  DRS000989
sample comment  B6 females were mated with JF1 males to generate [B6xJF1]F1 mice. Total RNA was extracted using ISOGEN (Nippon gene, Tokyo, Japan) and further purified RNeasy mini Kit and RNase-free DNase (Qiagen, CA, USA) from four [B6xJF1]F1 placentas at E13.5, and pooled. Twelve micrograms of total RNA was used for rRNA depletion (RiboMinus Eukaryote kit for RNA-seq, Invitrogen, CA, USA) and RNA-seq library construction (SOLiD Whole Transcriptome Analysis Kit, Life technologies, CA, USA) according to the manufacturer’s protocol.
bioproject  PRJDA72519
sra-run  DRR001435
sra-submission  DRA000448
sra-study  DRP000457
sra-sample  DRS000989
sra-experiment  DRX000989
distribution JSONJSON-LD
ddbj_biosample_set.xml.gz  HTTPS FTP
status public
visibility unrestricted-access
dateCreated 2014-05-12T01:17:49+09:00
dateModified 2014-11-12T08:28:28+09:00
datePublished 2012-04-09T15:00:00+09:00