home > biosample > SAMD00010730
identifier SAMD00010730
type biosample
sra-sample  DRS002605
organism Bacillus subtilis
sample_name  DRS002605
sample comment  Chromatin immunoprecipitation (ChIP) is a common method to map protein-binding sites in vivo. Combining this method with microarray (ChIP-chip) or high-throughput sequencing (ChIP-seq) allows us to detect protein-binding sites scattered across the entire genome. An enduring challenge for these methods, however, is how to increase their positional resolution. Here, we describe a new method, “Genome Footprinting by high-throughput sequencing (GeF-seq)”, to attain high-resolution mapping of protein-binding sites by combining in vivo DNase I digestion and ChIP-seq. We used GeF-seq to determine the binding site of Bacillus subtilis transition state regulator, AbrB, across the genome.
bioproject  PRJDB675
sra-run  DRR003185
sra-submission  DRA000758
sra-study  DRP000789
sra-sample  DRS002605
sra-experiment  DRX002517
distribution JSONJSON-LD
status public
visibility unrestricted-access
dateCreated 2014-05-12T01:17:49+0000
dateModified 2014-11-12T08:28:28+0000
datePublished 2013-03-31T15:00:00+0000