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| sample_name |
DRS002605 |
| sample comment |
Chromatin immunoprecipitation (ChIP) is a common method to map protein-binding sites in vivo. Combining this method with microarray (ChIP-chip) or high-throughput sequencing (ChIP-seq) allows us to detect protein-binding sites scattered across the entire genome. An enduring challenge for these methods, however, is how to increase their positional resolution. Here, we describe a new method, “Genome Footprinting by high-throughput sequencing (GeF-seq)”, to attain high-resolution mapping of protein-binding sites by combining in vivo DNase I digestion and ChIP-seq. We used GeF-seq to determine the binding site of Bacillus subtilis transition state regulator, AbrB, across the genome. |
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