| attributes |
| sample_name |
DRS002677 |
| strain |
B-276 |
| sample comment |
Bacteria and growth conditions: Cultures of R. corallinus B-276 (ATCC31338) were grown on Nutrient Broth No. 2 (Oxoid, Basingstoke, Hampshire, U.K.) containing 1% (w/v) glucose (NBG) at 30 °C with aerobic shaking (NSC). For the SC, propene and carbon were supplied via the gas phase with incubation in the NBG media at 30 °C. Cultures were grown either in shaking flasks or in sealed shaking flasks with propene (10%, v/v) supplied via the gas phase. Cells were harvested by centrifugation (8,000 × g for 10 min), washed twice with sterilized water, followed by re-centrifugation (8,000 × g for 10 min) to obtain cell pellets for DNA extraction. Cell pellets were stored at −80°C until use. |
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