home > biosample > SAMD00012509
identifier SAMD00012509
type biosample
sra-sample  DRS001598
organism Homo sapiens
sample_name  DRS001598
sample comment  This sample is total RNA that was treated BRIC in the control HeLa cells.It was harvested in 8h time point.Sequencing analysis was carried out without polyA selection.The siRNA duplexes were used at a final concentration of 10 nM and were transfected into cells using Lipofectamine RNAiMAX (Invitrogen), according to the manufacturer’s instructions. BRIC was performed basically as described. In brief, cells were incubated at 37°C in the presence of 150 μM 5'-bromo-uridine (BrU)for 24 h in a humidified incubator with 5% CO2. At indicated time points after the replacing of BrU-containing medium with BrU-free medium, cells were harvested for RNA preparation using RNAiso Plus, and total RNA was isolated. Twelve micrograms of BrU-labeled total RNA was denatured by heating at 80°C for 1 min and then added to the anti-BrdU mAb-conjugated beads, which contain 2 μg of the anti-BrdU mAb (clone 2B1, MBL). The mixture was incubated at room temperature for 1 h with rotation. Beads were washed 3 times with 0.1% BSA in PBS, and ISOGEN LS was added, followed by RNA isolation in accordance with the manufacturer’s instructions. The isolated RNA was used for RT-qPCR or deep sequencing.
bioproject  PRJDB2542
sra-run  DRR002255
sra-submission  DRA000591
sra-study  DRP000622
sra-sample  DRS001598
sra-experiment  DRX001673
distribution JSONJSON-LD
ddbj_biosample_set.xml.gz  HTTPS FTP
status public
visibility unrestricted-access
dateCreated 2014-05-12T01:17:49+0000
dateModified 2014-11-12T08:28:28+0000
datePublished 2013-03-26T15:00:00+0000