home > biosample > SAMD00016619
identifier SAMD00016619
type biosample
sra-sample  DRS000201
organism microbial mat metagenome
sample_name  DRS000201
sample comment  This is a metagenomic project to figure out the microbial community flourished along with hot water stream in the Japanese subsurface mine. As a first step of this project, we constructed a fosmid library from the DNA directly isolated from the microbial mat without cultivation. Since we could get about 3,500 fosmid clones, we determined the paired-end sequence of all fosmid clones. We selected putative archaeal genome fragments from the metagenomic library. Total of 132 fosmids were grouped into each 13 or 20 fosmid and each group was analyzed in a half plate of 454 GS20. In addition, 18 fosmids were analyzed by Sanger sequencing. A composite archaeal genome was reconstructed from 91 complete or partial fosmid sequences.
bioproject  PRJNA39577
sra-run  DRR000358
sra-submission  DRA000160
sra-study  DRP000160
sra-sample  DRS000201
sra-experiment  DRX000195
distribution JSONJSON-LD
ddbj_biosample_set.xml.gz  HTTPS FTP
status public
visibility unrestricted-access
dateCreated 2014-05-12T01:17:49+09:00
dateModified 2014-11-12T08:28:28+09:00
datePublished 2010-12-05T15:00:00+09:00