| attributes |
| sample_name |
Lachnospiraceae bacterium NBRC 112778 |
| bioproject_id |
PRJDB6766 |
| biotic_relationship |
free living |
| collection_date |
2017-01-06 |
| culture_collection |
NBRC:112778 |
| env_broad_scale |
freshwater biome |
| env_local_scale |
methane fermentation tank |
| env_medium |
water |
| estimated_size |
4500000 |
| geo_loc_name |
Japan:Fukushima, Date |
| isol_growth_condt |
not provided |
| lat_lon |
37.76125 N 140.57864 E |
| locus_tag_prefix |
lbkm |
| num_replicons |
1 |
| project_name |
New bacteria whole genome sequencing, isolated from methane fermentator |
| ref_biomaterial |
not provided |
| samp_mat_process |
This bacterium was originally isolated from a mesophilic methanogenic bioreactor digesting sweet potato. Methanogenic sludge was inoculated onto an agar plate of RCM (reinforced clostridial medium) containing Red Starch as an indicator for amylase (grams per liter): yeast extract, 13; peptone, 10; glucose, 5; Red Starch, 1; NaCl, 5; sodium acetate, 3, Cysteine-HCl 0.5 (pH6.8) and anaerobically incubated at 37 degrees C for three days using AnaeroPack pouch. Colonies forming clear halo were isolated and purified by successive dilution and cultivation in the same condition. For pure cultivation, RCM containing 0.1 % normal soluble starch was used. |
| strain |
KM106-2 |
| trophic_level |
heterotroph |
|