ENA first public |
2016-02-06 |
ENA last update |
2016-10-21 |
ENA-CHECKLIST |
ERC000011 |
External Id |
SAMEA3697716 |
INSDC center alias |
HELMHOLTZ CENTRE FOR ENVIRONMENTAL RESEARCH - UFZ. |
INSDC center name |
HELMHOLTZ CENTRE FOR ENVIRONMENTAL RESEARCH - UFZ. |
INSDC first public |
2016-02-06T17:05:42Z |
INSDC last update |
2016-10-21T09:49:07Z |
INSDC status |
public |
Submitter Id |
135 |
amount or size of sample collected |
15 |
base saturation |
17.75 |
carbon/nitrogen |
14.2 |
cation exchange capacity |
54.58 |
collected by |
Zhiqin Pei and Christina Weissbecker |
collection date |
2011-10 |
composite design/sieving (if any) |
one forest plot had the size 25.8 m x 25.8 m with 400 tree individuals planted in 1.29 m distance, at each forest plot four tree individuals of each tree species were randomly selected for sampling, at each sampling tree four soil cores were retrieved at 20-30 cm distance in each direction, all four cores form one composite sample for each sampling tree |
depth |
0.1 |
diversity level |
2 |
dna extraction kit |
rewetting of 1 g soil with 5 ml clean water five min prior to extraction,RNA Power Soil Total RNA Isolation kit followed by RNA Power Soil DNA Elution Accessory kit (Mobio) |
environment (biome) |
forest |
environment (feature) |
subtropical experimental forest |
environment (material) |
soil |
environmental package |
soil |
experimental factor |
diversity gradient |
geographic location (country and/or sea) |
China |
geographic location (elevation) |
131.93 |
geographic location (latitude) |
29.12156593 |
geographic location (longitude) |
117.9057028 |
geographic location (region and locality) |
Jiangxi Province, Xingangshan village |
history/previous land use |
Cunninghamia lanceolata plantation |
investigation type |
mimarks-survey |
iron exchange capacity |
0.1 |
mycorrhiza type sampling tree |
EcM |
note |
soil |
ph_h2o |
4.61 |
plot id |
C32 |
sample collection device or method |
metal soil corer of 6 cm diameter and 10 cm height |
sample material processing |
removal of roots and stones,sieving 2 mm mesh, homogenization of 4 soil cores collected around one sampling tree, snap-freezing of about 15g soil in liquid nitrogen, transport on dry ice and storage at -80�C, freeze-drying before transport and relocation to -80�C upon arrival four days later |
sample name |
135 |
sample weight for DNA extraction |
1 |
sampling tree |
Castanea_henryi |
slope |
39.88 |
soil taxonomic/fao classification |
Cambisol |
storage conditions (fresh/frozen/other) |
freeze-dried for transportation and long-term storage at -80�C |
total organic carbon |
3.25 |
url |
http://www.bef-china.de |
water content |
38.25 |