home > biosample > SAMEA4415615
identifier SAMEA4415615
type biosample
sameAs
sra-sample  ERS1327064
organism Mus musculus
attributes
ArrayExpress-CellType  TS-Rs26
ArrayExpress-Species  Mus musculus
ENA first public  2017-01-24
ENA last update  2018-02-08
ENA-CHECKLIST  ERC000011
External Id  SAMEA4415615
INSDC center alias  SC
INSDC center name  Wellcome Sanger Institute
INSDC first public  2017-01-24T13:33:27Z
INSDC last update  2018-02-08T16:37:44Z
INSDC status  public
Submitter Id  18e54d60-4d97-11e6-9a3d-3c4a9275d6c8
common name  house mouse
sample description  Total RNA from a single confirmed Nubpl knockout clone generated and grown in parallel to vector control clones was extracted using TRIzol reagent. Cells were plated at 20,000 cells/6-well well. The RNA sample is a pool of 2 6-well wells grown in parallel.Total RNA from a single vector control clone generated and grown in parallel to Crb2 HOMs clones was prepared as described above. Cells were plated at 20,000 cells/6-well well. The RNA sample is a pool of 2 6-well wells grown in parallel. Cells were plated in TSC conditions, media changed to Differentiation conditions the next day, and cells harvested 3 days later, i.e. 3D of differentiation. The Nubpl CRISPR knockout design deletes exon 2.
sample name  18e54d60-4d97-11e6-9a3d-3c4a9275d6c8
properties 
{...}
dbXrefs
bioproject  PRJEB14846
sra-run  ERR1805580ERR1805626
sra-submission  ERA791923
sra-study  ERP016523
sra-sample  ERS1327064
sra-experiment  ERX1868607ERX1868653
distribution JSONJSON-LD
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biosample_set.xml.gz  HTTPS FTP
status public
visibility unrestricted-access
dateCreated 2017-01-25T12:05:55Z
dateModified 2021-08-21T13:24:07Z
datePublished 2017-01-24T00:00:00Z