| ENA first public |
2018-05-27 |
| ENA last update |
2018-05-13 |
| ENA-CHECKLIST |
ERC000037 |
| External Id |
SAMEA4662975 |
| INSDC center alias |
Department of Biochemistry and Molecular Biology and Institute of Bioinformatics |
| INSDC center name |
Department of Biochemistry and Molecular Biology and Institute of Bioinformatics |
| INSDC first public |
2018-05-27T17:02:47Z |
| INSDC last update |
2018-05-13T01:07:51Z |
| INSDC status |
public |
| Submitter Id |
Switchgrass PacBio non-FL transcript |
| collection date |
2014 |
| common name |
switchgrass |
| estimated size |
11.35G |
| geographic location (country and/or sea) |
USA |
| geographic location (latitude) |
40 |
| geographic location (longitude) |
30 |
| isolation and growth condition |
Total RNA of the above ground tissues was isolated using RNeasy Plant Mini Kit. Total RNA of roots was isolated from roots using TRI REAGENT |
| number of replicons |
1 |
| plant developmental stage |
R1 developmental stage |
| plant growth medium |
a 16-h light photoperiod (6:00 am-10:00 pm) with supplementary lighting from parabolic aluminized reflector lamps (average 390 μE/m2/S1), and relative humidity 77-22% (average 51%). The temperature in the greenhouse ranged from 25°C to 29 °C (average 26 °C). Plants were watered three times per week, and fertilizer (Peter’s Fert 20-10-20, 100 ppm) was applied during the last watering each week. |
| plant structure |
A whole tiller consisting of leaves, leaf sheaths, internodes, nodes and flowers and roots were collected |
| sample name |
Switchgrass PacBio non-FL transcript |