env_package |
MIGS/MIMS/MIMARKS.host-associated |
investigation_type |
metagenome |
label |
swine |
biome |
swine intestinal tract |
collection_date |
2010-01-12 |
feature |
none |
geo_loc_name |
USA: Ames, Iowa |
lat_lon |
42°02?05?N 93°37?12?W |
material |
contents of the distal ileum, proximal to the ileal/cecal junction |
project_name |
Swine gut microbial ecology along the gut transect |
samp_mat_process |
blend 5 g material in PBS, centrifuge at 250xg to reduce plant material, then centrifuge the supernatant at 10,000g to pellet the cells, followed by bead beating the resuspended pellet for bulk DNA extraction (kit by MoBio) |
specific_host |
Pig 65 |
host_common_name |
pig |
host_growth_cond |
ambient |
host_taxid |
9825 |
perturbation |
medicated (15 continuous days) |
samp_size |
up to 5 grams feces/gut contents |
samp_store_dur |
1 to 6 months |
samp_store_temp |
-20 degrees Celsius |
host_age |
92 days old |
host_body_product |
intestinal contents |
host_tissue_sampled |
ileum |
host_diet |
TechStart 17-25 (Kent Feeds, Muscatine, IA) containing ASP250 (chlortetracycline 100 g/ton, sulfamethazine 100 g/ton, penicillin 50 g/ton) ad libitum until euthanization (15 continuous days) |
host_family_relationship |
offspring of sow C |
host_last_meal |
not fasted before euthanasia |
host_life_stage |
grower pig |
host_sex |
female |