| attributes |
| strain |
ANKA (cl15cy1) |
| dev_stage |
Ookinete |
| sample_type |
PolyA+ RNA |
| cell_type |
mosquito stage |
| culture conditions |
Synchronized asexual infections were established in Wistar rats by intravenous injection of purified mature schizonts (time-point 0 hour), resulting in highly synchronous infections of 1-3% parasitemia (Janse and Waters, Parasitol. Today, 1995). 4 hours after injection of schizonts, infected blood (containing rings 0-4 hours post-invasion) was collected, leukocytes were removed and parasites were further cultured at 37°C under standard in vitro conditions (Janse and Waters, Parasitol. Today, 1995). Rings, trophozoites and schizonts were collected 4, 16 and 22 hours after injection of schizonts. Mature gametocyte populations were obtained by treatment of P. berghei infected mice with sulfadiazine and collected from the mice by heart puncture, after which leukocytes were removed and gametocytes were purified using Nycodenz gradient centrifugation (Beetsma, van der Wiel, Sauerwein. Eling, Exp. Parasitol, 1998). Ookinetes were producted from the purified gametocyte cultures by standard in vitro culture for ookinete production at 21C for 22 hours (Janse et al., Parasitology, 1985). ; |
|