identifier |
DRS019019 |
type |
sra-sample |
sameAs |
|
organism |
Mus musculus
|
attributes |
strain |
C57BL/6 X ICR |
sample_title |
F2C_3 |
host_body_site |
fertilized 2-cell embryos |
description |
Total RNA and polyA+ RNA were extracted from pools, each of which contained one hundred MII oocytes or 2-cell embryos (C57BL/6 X ICR), by using a Dynabeads mRNA DIRECT Micro Kit (Invitrogen). Four replicates were made for directional RNA-seq library construction by using an NEBNext Ultra Directional RNA Library Prep Kit for Illumina (NEB). In this library preparation, cDNAs were enriched by 15-cycle PCR. Illumina HiSeq 2000 was used to perform 50-bp single-end sequencing according to the manufacturer?fs instructions. |
isolate |
Dynabeads |
sample_name |
F2C_3 |
bioproject_id |
PRJDB2994 |
|
properties ▽ |
{...}
|
dbXrefs |
|
distribution |
JSONJSON-LD
|
Download |
|
status |
public |
visibility |
unrestricted-access |
dateCreated |
2014-07-22T02:12:05+09:00 |
dateModified |
2015-02-21T22:37:14+09:00 |
datePublished |
2015-02-21T22:37:14+09:00 |