| identifier |
DRS019028 |
| type |
sra-sample |
| sameAs |
|
| organism |
Mus musculus
|
| attributes |
| strain |
C57BL/6 X ICR |
| sample_title |
siC_morula_4 |
| description |
Total RNA and polyA+ RNA were extracted from pools, each of which contained one hundred MII oocytes or 2-cell embryos (C57BL/6 X ICR), by using a Dynabeads mRNA DIRECT Micro Kit (Invitrogen). Four replicates were made for directional RNA-seq library construction by using an NEBNext Ultra Directional RNA Library Prep Kit for Illumina (NEB). In this library preparation, cDNAs were enriched by 15-cycle PCR. Illumina HiSeq 2000 was used to perform 50-bp single-end sequencing according to the manufacturer's instructions. |
| isolate |
Dynabeads |
| sample_name |
siC_morula_4 |
| bioproject_id |
PRJDB2994 |
|
| properties ▽ |
{...}
|
| dbXrefs |
|
| distribution |
JSONJSON-LD
|
| Download |
|
| status |
public |
| visibility |
unrestricted-access |
| dateCreated |
2014-07-22T02:12:05+09:00 |
| dateModified |
2015-02-21T22:37:14+09:00 |
| datePublished |
2015-02-21T22:37:14+09:00 |