| identifier |
DRS023187 |
| type |
sra-sample |
| sameAs |
|
| organism |
Caenorhabditis elegans
|
| attributes |
| strain |
N2 |
| sample_title |
N2 L1 [RNAseq] |
| genotype |
wild type |
| description |
The worm strain used was N2. This strain is wild type. Total RNAs were extracted from synchronized L1 worms by using RNeasy Midi kit (Qiagen). Poly (A)+ RNAs were repeatedly purified with FastTrack MAG (Invitrogen), fragmented in 1?~ Fragmentation buffer (Ambion) and reverse transcribed using random hexamers and Primescript II (Takara). Second strand cDNAs were synthesized by using RNase H (Invitrogen) and DNA polymerase I (Invitrogen). Deep sequencing was performed by utilizing Genome Analyzer II (Illumina) following standard protocols. |
| sample_name |
N2 L1 |
| bioproject_id |
PRJDB2743 |
| dev_stage |
L1 |
|
| properties ▽ |
{...}
|
| dbXrefs |
|
| distribution |
JSONJSON-LD
|
| Download |
|
| status |
public |
| visibility |
unrestricted-access |
| dateCreated |
2014-10-17T06:15:21+09:00 |
| dateModified |
2016-01-28T22:03:27+09:00 |
| datePublished |
2016-01-28T22:03:27+09:00 |