| attributes |
| description |
Cells from one 10 cm dish (~1 x 107) of HEK293T cells grown to 70-80% confluence were used for each immunoprecipitation. The cells were cross-linked with 2 mM DSG Crosslinker in PBS for 30 min and then 1% formaldehyde in PBS for 20 min at room temperature. Then the cells were resuspended and lysed in lysis buffer (0.5% SDS, 10mM EDTA, 150 mM NaCl, 50 mM Tris-HCl pH 8.0), and they were sonicated with a Bioruptor Sonicator (Diagenode, Denville, NJ) for 30 times for 30 seconds at the maximum power setting to generate DNA fragments of ~150-500 bps. Sonicated chromatin was incubated at 4C overnight with 10 ug of anti-TAF7 (SQ-8, sc-101167, Santa cruz). Crosslinking was reversed by overnight incubation at 65C. Immunoprecipitated DNA were treated with RNase A and Proteinase K by incubation at 45C. DNA was purified using the QIAquick MinElute PCR purification kit (28006, Qiagen). |
| bioproject_id |
PRJDB3302 |
| cell_line |
HEK293T |
| sample_name |
TAF7 |
| sample_title |
HEK293T_TAF7_ChIPseq |
|