identifier |
DRS085402 |
type |
sra-sample |
sameAs |
|
organism |
Saccharomyces cerevisiae BY4741
|
attributes |
strain |
YTT157 |
replicate |
biological replicate 2 |
mating_type |
MATa |
description |
Replicate sample_2. Parental yeast strain with low rDNA copy, YPD culture cross-linked by 1% formaldehyde at 30degree Celsius for 30 minutes. 2x10^9 cells were lysed by YTZ-beads, and the extracts were sheared by Covaris S220 to 100bp average. After pelleting, soluble fractions were used for immunoprecipitation with 10micro-g of anti-HA F-7 antibody and ProteinG dynabeads. Immunoprecipitates were reverse-crosslinked and purified. Sequencing libraries were prepared by KAPA Hyperprep kit and TruSeqHT-compatible adaptors. |
bioproject_id |
PRJDB7030 |
sample_title |
WT_RDN1-15_IP2 |
genotype |
MATa fob1Δ0 RDN1-15 his3Δ1 leu2Δ0 met15Δ0 ura3Δ0 |
sample_name |
UAF_ChIP008 |
|
properties ▽ |
{...}
|
dbXrefs |
|
distribution |
JSONJSON-LD
|
Download |
|
status |
public |
visibility |
unrestricted-access |
dateCreated |
2016-01-13T07:36:44+09:00 |
dateModified |
2019-01-03T22:05:56+09:00 |
datePublished |
2019-01-03T22:05:56+09:00 |