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identifier DRS085403
type sra-sample
sameAs
organism Saccharomyces cerevisiae BY4741
attributes
strain  YTT213
replicate  biological replicate 1
mating_type  MATa
description  Replicate sample_1. UAF30-MN3HA tagged yeast strain with normal rDNA copy, YPD culture cross-linked by 1% formaldehyde at 30degree Celsius for 30 minutes. 2x10^9 cells were lysed by YTZ-beads, and the extracts were sheared by Covaris S220 to 100bp average. After pelleting, soluble fractions were used for INPUT samples. After reverse-crosslinking and DNA purification, sequencing libraries were prepared by KAPA Hyperprep kit and TruSeqHT-compatible adaptors.
bioproject_id  PRJDB7030
sample_title  UAF30-MN3HA_RDN1-150_INPUT1
genotype  MATa UAF30-MN3HA::natMX fob1Δ0 RDN1-150 his3Δ1 leu2Δ0 met15Δ0 ura3Δ0
sample_name  UAF_ChIP009
properties 
{...}
dbXrefs
bioproject  PRJDB7030
sra-run  DRR131077
sra-submission  DRA006859
biosample  SAMD00119017
sra-study  DRP004716
sra-experiment  DRX123815
distribution JSONJSON-LD
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DRA006859.sample.xml  HTTPS FTP
status public
visibility unrestricted-access
dateCreated 2016-01-13T07:36:44+09:00
dateModified 2019-01-03T22:05:56+09:00
datePublished 2019-01-03T22:05:56+09:00