| attributes |
| description |
Cerebellums (50-90 mg) from controls mice aged 4 weeks were used to extract total RNAs. RNA was isolated with Sepasol-RNA I Super G (Nacalai Tesque, Kyoto, Japan) according to the manufacturer's instructions. All libraries were prepared using the TruSeq RNA Sample Preparation Kits v2 (Illumina, San Diego, CA USA) according to the manufacturer's instructions. In brief, magnetic beads containing poly-dT molecules were first used to purify mRNA from 250 ng of total RNA. Second, samples were chemically fragmented and reverse transcribed into cDNA. Finally, end repair and A-base tailing was performed before Illumina adapters were ligated to the cDNA fragments. Purified samples were amplified by 15-cycle PCR. Amplified material was validated and quantified using an Agilent 2100 bioanalyzer and the DNA 1000 Nano Chip Kit (Agilent, Technologies, Santa Clara, CA, USA). |
| bioproject_id |
PRJDB3340 |
| sample_name |
PC_cont_4w RNAseq |
| phenotype |
wild type |
| strain |
Jsap1flox/flox:Jlpflox/flox (backcrossed for ten generations onto a C57BL/6 background) |
| sample_title |
PC_cont_4w RNAseq |
| genotype |
floxed Jsap1:Jlp without GluRdelta2-Cre |
| tissue_type |
cerebellum |
| age |
4 weeks |
|