| Alias |
C_000154 |
| Description |
16S rRNA sequences of fecal bacterial community from Yeongsan river site Y3 |
| INSDC center alias |
GIST |
| INSDC center name |
Gwangju Institute of Science and Technology, South Korea |
| INSDC first public |
2010-11-08T08:45:40Z |
| INSDC last update |
2018-03-09T09:52:22Z |
| INSDC status |
public |
| SRA accession |
ERS009010 |
| Sample Name |
ERS009010 |
| Sample storage condition |
-80 |
| Sample storage duration |
3 months |
| Title |
16S rRNA sequences of fecal bacterial community from Yeongsan river site Y3 |
| amount or size of sample collected |
50 |
| collection date |
2008 |
| geographic location (Country) |
South Korea:Damyang, Yeongsan river |
| health or disease status of specific host at time of collection |
>1600 E. coli CFU/ 100ml |
| library reads sequenced |
29101 |
| nucleic acid amplification |
PCR |
| nucleic acid extraction |
Stool DNA extraction kit (Bioneer, South Korea) |
| pcr conditions |
initial denaturation at 94C for 5 min; followed by 25 cycles of denaturation at 94C for 30 sec, annealing at 60C for 30 sec, and elongation at 72C for 1 min 20 sec. The amplified PCR products were purified using resin columns, and 1 microgram of PCR product for each sample was pooled and subjected to pyrosequencing. |
| pcr primers |
(5'-X-AC-GAGTTTGATCMTGGCTCAG-3' and 5'-X-AC-WTTACCGCGGCTGCTGG-3' where X denotes a barcode uniquely designed for each sample with a linker AC |
| sample collection device or method |
freeze dry |
| sequencing method |
454 GS FLX |
| target gene |
16S 27F 518R |