Alias |
C_000141 |
Description |
16S rRNA sequences of fecal bacterial community from Dairy cattle |
INSDC center alias |
GIST |
INSDC center name |
Gwangju Institute of Science and Technology, South Korea |
INSDC first public |
2010-11-08T08:45:40Z |
INSDC last update |
2018-03-09T09:52:22Z |
INSDC status |
public |
SRA accession |
ERS009014 |
Sample Name |
ERS009014 |
Sample storage condition |
-80 degree C |
Sample storage duration |
3 months |
Title |
16S rRNA sequences of fecal bacterial community from Dairy cattle |
amount or size of sample collected |
200 |
collection date |
2008 |
geographic location (Country) |
South Korea:Jeonnam, farm |
health or disease status of specific host at time of collection |
healthy |
host common name |
Milk cow |
host tax-id |
9913 |
library reads sequenced |
6593 |
nucleic acid amplification |
PCR |
nucleic acid extraction |
Stool DNA extraction kit (Bioneer, South Korea) |
pcr conditions |
initial denaturation at 94C for 5 min; followed by 25 cycles of denaturation at 94C for 30 sec, annealing at 60C for 30 sec, and elongation at 72C for 1 min 20 sec. The amplified PCR products were purified using resin columns, and 1 microgram of PCR product for each sample was pooled and subjected to pyrosequencing. |
pcr primers |
(5'-X-AC-GAGTTTGATCMTGGCTCAG-3' and 5'-X-AC-WTTACCGCGGCTGCTGG-3' where X denotes a barcode uniquely designed for each sample with a linker AC |
sample collection device or method |
fecal swab |
sequencing method |
454 GS FLX |
target gene |
16S 27F 518R |