Alias |
C_000153 |
Description |
16S rRNA sequences of fecal bacterial community from Yeongsan river site Y2 |
INSDC center alias |
GIST |
INSDC center name |
Gwangju Institute of Science and Technology, South Korea |
INSDC first public |
2010-11-08T08:45:41Z |
INSDC last update |
2018-03-09T10:03:12Z |
INSDC status |
public |
SRA accession |
ERS009018 |
Sample Name |
ERS009018 |
Sample storage condition |
-80 |
Sample storage duration |
3 months |
Title |
16S rRNA sequences of fecal bacterial community from Yeongsan river site Y2 |
amount or size of sample collected |
50 |
collection date |
2008 |
geographic location (Country) |
South Korea:Gwangju, Yeongsan river |
health or disease status of specific host at time of collection |
>1600 E. coli CFU/ 100ml |
library reads sequenced |
10857 |
nucleic acid amplification |
PCR |
nucleic acid extraction |
Stool DNA extraction kit (Bioneer, South Korea) |
pcr conditions |
initial denaturation at 94 C for 5 min; followed by 25 cycles of denaturation at 94 C for 30 sec, annealing at 60 C for 30 sec, and elongation at 72 C for 1 min 20 sec. The amplified PCR products were purified using resin columns, and 1 g of PCR product for each sample was pooled and subjected to pyrosequencing. |
pcr primers |
(5'-X-AC-GAGTTTGATCMTGGCTCAG-3' and 5'-X-AC-WTTACCGCGGCTGCTGG-3' where X denotes a barcode uniquely designed for each sample with a linker AC |
sample collection device or method |
freeze dry |
sequencing method |
454 GS FLX |
target gene |
16S 27F 518R |