| attributes |
| Alias |
E-MTAB-434:ZF_2cells |
| Broker name |
ArrayExpress |
| Description |
Protocols: Zebrafish embyos or tissues were collected from a Tuebingen strain incross and grown at 28 C. Collected samples were snap frozen on dry ice and stored at -70 C Total RNA was extracted using Trizol Reagent (Invitrogen) following the manufacturer's instructions. Pellets were resuspended RNase-free 10 mM Tris pH 7.5 and the RNA was quantified using a NanoDrop ND-1000 Spectrophotometer (Axon Instruments). Total RNA was made into an RNAseq Illumina library following the manufacturer's protocol including a DNase treatment between to 2 rounds of polyA pull down. The libraries have fragment size of 250 to 300 bp. |
| DevelopmentalStage |
embryo |
| INSDC center alias |
SC |
| INSDC center name |
Wellcome Sanger Institute |
| INSDC first public |
2011-03-10T17:55:05Z |
| INSDC last update |
2018-03-08T15:25:14Z |
| INSDC status |
public |
| SRA accession |
ERS017427 |
| Sample Name |
ERS017427 |
| Sex |
mixed |
| StrainOrLine |
Tuebingen |
| Title |
ZF_2cells |
|