attributes |
ENA first public |
2012-12-31 |
ENA last update |
2018-03-09 |
ENA-KEYWORD |
GSC, MIxS, MIMARKS |
External Id |
SAMEA1684145 |
GSC:MIMARKS:v2.1 |
Compliant |
Geographic location (country:region,area) |
Finland, Southern Finland |
INSDC center alias |
UH-FS |
INSDC center name |
Forest pathology lab, Department of Forest Sciences, University of Helsinki, Finland |
INSDC first public |
2012-12-31T17:01:33Z |
INSDC last update |
2018-03-09T09:53:43Z |
INSDC status |
public |
Submitter Id |
CLO3 |
amount or size of sample collected |
5 - 10 |
chemical administration |
none (control) |
collection date |
40313 |
depth |
0.0-0.02 |
elevation |
0 |
enviornmental package |
miscellaneous natural or artificial environment |
environment (biome) |
forest |
environment (feature) |
wood |
environment (material) |
wood stump in forest |
experimental factor |
time |
geographic location (latitudeandlongitude) |
60 N 23 E |
investigation type |
MIMARKS_Survey |
miscellaneous parameter |
Tree species of stump:Norway Spruce |
miscellaneous parameter |
Time elapsed since felling: 6 years |
nucleic acid extraction |
Genomic DNA was extracted from 500 mg of the homogenized wood sample using the 'PowerSoilTM DNA Isolation Kit' (MoBio Laboratories, Carlsbad, CA, USA). |
plant body site |
stem base (dead tree stump) |
project name |
Pg effect |
sample collection device or method |
five 1x1x2 cm cores, pooled |
sample name |
CLO3 |
sample weight for DNA extraction |
0.5 |
sequencing method |
Pyrosequencing |
sequencing method |
pyrosequencing |
target gene |
16S rRNA |
target subfragment |
V1-V3 regions |
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