| attributes |
| ENA first public |
2012-12-31 |
| ENA last update |
2018-03-09 |
| ENA-KEYWORD |
GSC, MIxS, MIMARKS |
| External Id |
SAMEA1684154 |
| GSC:MIMARKS:v2.1 |
Compliant |
| Geographic location (country:region,area) |
Finland, Southern Finland |
| INSDC center alias |
UH-FS |
| INSDC center name |
Forest pathology lab, Department of Forest Sciences, University of Helsinki, Finland |
| INSDC first public |
2012-12-31T17:01:33Z |
| INSDC last update |
2018-03-09T09:53:43Z |
| INSDC status |
public |
| Submitter Id |
TS2 |
| amount or size of sample collected |
5 - 10 |
| chemical administration |
biocontrol agent (Phlebiopsis gigantea), applied to stump at time of felling |
| collection date |
40313 |
| depth |
0.0-0.02 |
| elevation |
0 |
| enviornmental package |
miscellaneous natural or artificial environment |
| environment (biome) |
forest |
| environment (feature) |
wood |
| environment (material) |
wood stump in forest |
| experimental factor |
Time |
| geographic location (latitudeandlongitude) |
60 N 28 E |
| investigation type |
MIMARKS_Survey |
| miscellaneous parameter |
Tree species of stump:Norway Spruce |
| miscellaneous parameter |
Time elapsed since felling: 13 years |
| nucleic acid extraction |
Genomic DNA was extracted from 500 mg of the homogenized wood sample using the 'PowerSoilTM DNA Isolation Kit' (MoBio Laboratories, Carlsbad, CA, USA). |
| plant body site |
stem base (dead tree stump) |
| project name |
Pg effect |
| sample collection device or method |
five 1x1x2 cm cores, pooled |
| sample name |
TS2 |
| sample weight for DNA extraction |
0.5 |
| sequencing method |
Pyrosequencing |
| sequencing method |
pyrosequencing |
| target gene |
16S rRNA |
| target subfragment |
V1-V3 regions |
|