| ENA first public |
2016-02-06 |
| ENA last update |
2016-10-21 |
| External Id |
SAMEA3697810 |
| INSDC center alias |
HELMHOLTZ CENTRE FOR ENVIRONMENTAL RESEARCH - UFZ. |
| INSDC center name |
HELMHOLTZ CENTRE FOR ENVIRONMENTAL RESEARCH - UFZ. |
| INSDC first public |
2016-02-06T17:05:42Z |
| INSDC last update |
2016-10-21T09:49:09Z |
| INSDC status |
public |
| Submitter Id |
234 |
| amount or size of sample collected |
15 |
| base saturation |
38.71 |
| carbon/nitrogen |
13.63 |
| cation exchange capacity |
51.1 |
| collected by |
Zhiqin Pei and Christina Weissbecker |
| collection date |
2011-10 |
| composite design/sieving (if any) |
one forest plot had the size 25.8 m x 25.8 m with 400 tree individuals planted in 1.29 m distance, at each forest plot four tree individuals of each tree species were randomly selected for sampling, at each sampling tree four soil cores were retrieved at 20-30 cm distance in each direction, all four cores form one composite sample for each sampling tree |
| depth |
0.1 |
| diversity level |
4 |
| dna extraction kit |
rewetting of 1 g soil with 5 ml clean water five min prior to extraction,RNA Power Soil Total RNA Isolation kit followed by RNA Power Soil DNA Elution Accessory kit (Mobio) |
| environment (biome) |
forest |
| environment (feature) |
subtropical experimental forest |
| environment (material) |
soil |
| environmental package |
soil |
| experimental factor |
diversity gradient |
| geographic location (country and/or sea) |
China |
| geographic location (elevation) |
179.92 |
| geographic location (latitude) |
29.12275325 |
| geographic location (longitude) |
117.9064182 |
| geographic location (region and locality) |
Jiangxi Province, Xingangshan village |
| history/previous land use |
Cunninghamia lanceolata plantation |
| investigation type |
mimarks-survey |
| iron exchange capacity |
0.1 |
| mycorrhiza type sampling tree |
EcM |
| note |
soil |
| ph_h2o |
4.87 |
| plot id |
F27 |
| sample collection device or method |
metal soil corer of 6 cm diameter and 10 cm height |
| sample material processing |
removal of roots and stones,sieving 2 mm mesh, homogenization of 4 soil cores collected around one sampling tree, snap-freezing of about 15g soil in liquid nitrogen, transport on dry ice and storage at -80�C, freeze-drying before transport and relocation to -80�C upon arrival four days later |
| sample name |
234 |
| sample weight for DNA extraction |
1 |
| sampling tree |
Quercus_serrata |
| slope |
33.92 |
| soil taxonomic/fao classification |
Cambisol |
| storage conditions (fresh/frozen/other) |
freeze-dried for transportation and long-term storage at -80�C |
| total organic carbon |
2.46 |
| url |
http://www.bef-china.de |
| water content |
34.82 |