| ENA first public |
2018-01-05 |
| ENA last update |
2017-05-11 |
| External Id |
SAMEA4067784 |
| INSDC center alias |
Institute for Molecular Biosciences (UQ) |
| INSDC center name |
Institute for Molecular Biosciences (UQ) |
| INSDC first public |
2018-01-05T17:02:51Z |
| INSDC last update |
2017-05-11T06:44:19Z |
| INSDC status |
public |
| Submitter Id |
IMB_RL6_HCEVDL1 |
| amount or size of sample collected |
4 venom glands |
| broker name |
Bioinformatics Resources Australia - EMBL |
| collection date |
2012 |
| geographic location (country and/or sea) |
Australia |
| lat lon |
33.864174 S 151.20528680 E |
| sample material processing |
All spiders used for complementary DNA (cDNA) library construction were first anesthetized using CO2, frozen at –80°C for 10 min, and then dissected at 4°C. To access the venom gland, the chelicerae were first removed from the base of the structure (towards the carapace). Once separated, each chelicera was individually cut from the ventral side up to the base of the fang in order to approach the venom gland from underneath. The cheliceral muscle that surrounds the venom gland was detached to expose the isolated venom gland. Dissected venom glands were placed immediately in TRIzol® reagent (Invitrogen) to be processed. |
| sample name |
IMB_RL6_HCEVDL1 |