attributes |
ArrayExpress-CellType |
TS-Rs26 |
ArrayExpress-Species |
Mus musculus |
ENA first public |
2017-01-24 |
ENA last update |
2018-02-08 |
External Id |
SAMEA4415612 |
INSDC center alias |
SC |
INSDC center name |
Wellcome Sanger Institute |
INSDC first public |
2017-01-24T13:33:27Z |
INSDC last update |
2018-02-08T16:36:18Z |
INSDC status |
public |
Submitter Id |
18af4940-4d97-11e6-9a3d-3c4a9275d6c8 |
common name |
house mouse |
sample description |
Total RNA from a single confirmed Nubpl knockout clone generated and grown in parallel to vector control clones was extracted using TRIzol reagent. Cells were plated at 20,000 cells/6-well well. The RNA sample is a pool of 2 6-well wells grown in parallel.Total RNA from a single vector control clone generated and grown in parallel to Crb2 HOMs clones was prepared as described above. Cells were plated at 20,000 cells/6-well well. The RNA sample is a pool of 2 6-well wells grown in parallel. Cells were plated in TSC conditions, media changed to Differentiation conditions the next day, and cells harvested 3 days later, i.e. 3D of differentiation. The Nubpl CRISPR knockout design deletes exon 2. |
sample name |
18af4940-4d97-11e6-9a3d-3c4a9275d6c8 |
|