home > sra-sample > ERS1327070
identifier ERS1327070
type sra-sample
sameAs
biosample  SAMEA4415621
organism Mus musculus
attributes
ArrayExpress-CellType  TS-Rs26
ArrayExpress-Species  Mus musculus
ENA first public  2017-01-24
ENA last update  2018-02-08
External Id  SAMEA4415621
INSDC center alias  SC
INSDC center name  Wellcome Sanger Institute
INSDC first public  2017-01-24T13:33:27Z
INSDC last update  2018-02-08T16:41:18Z
INSDC status  public
Submitter Id  1954ff20-4d97-11e6-9a3d-3c4a9275d6c8
common name  house mouse
sample description  Total RNA from a single confirmed Crb2 knockout clone generated and grown in parallel to vector control clones was extracted using TRIzol reagent. Cells were plated at 20,000 cells/6-well well. The RNA sample is a pool of 2 6-well wells grown in parallel.Total RNA from a single vector control clone generated and grown in parallel to Crb2 HOMs clones was prepared as described above. Cells were plated at 20,000 cells/6-well well. The RNA sample is a pool of 2 6-well wells grown in parallel. Cells were plated in TSC conditions, media changed to Differentiation conditions the next day, and cells harvested 3 days later, i.e. 3D of differentiation. The Crb2 CRISPR knockout design deletes exons 7&8.
sample name  1954ff20-4d97-11e6-9a3d-3c4a9275d6c8
properties 
{...}
dbXrefs
bioproject  PRJEB14846
sra-run  ERR1805592ERR1805638
sra-submission  ERA791923
biosample  SAMEA4415621
sra-study  ERP016523
sra-experiment  ERX1868619ERX1868665
distribution JSONJSON-LD
Download
ERA791923.sample.xml  HTTPS FTP
status public
visibility unrestricted-access
dateCreated 2017-01-25T16:37:24Z
dateModified 2021-09-24T12:22:26Z
datePublished 2017-01-25T17:11:17Z