| identifier |
PRJDB9314 |
| type |
bioproject |
| sameAs |
|
| organism |
Nicotiana tabacum
|
| title |
Analysis of TAS3 tasiRNAs produced in a tobacco cell-free system. |
| description |
Many organisms have developed an RNA silencing amplification pathway, in which primary small RNAs trigger the production of secondary small interfering RNAs (siRNAs) from double-stranded RNAs that is converted by RNA-dependent RNA polymerase (RDR). This pathway is required for the regulation of endogenous gene expression as well as the defense against non-self-RNAs from viruses and transposons. However, the molecular mechanisms of the signal amplification pathway, especially how RDRs recognize their specific substrate RNAs and initiate complementary strand synthesis, are not well understood. Here, we recapitulated “TAS3 tasiRNA pathway”, an endogenous plant secondary siRNA biogenesis pathway, in a tobacco cell-free system. To look at the expression pattern of the secondary siRNAs in our system, we analyzed the small RNAs accumulated in the tobacco cell-free system after triggering the production of TAS3 tasiRNAs. Small RNA libraries were prepared using NEB Next Multiplex Small RNA Library Prep Set for Illumina (NEB), and analyzed by Illumina HiSeq 4000. |
| data type |
Transcriptome or Gene Expression
|
| publication |
|
| properties ▽ |
{...}
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| dbXrefs |
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| distribution |
JSONJSON-LD
|
| Download |
|
| status |
public |
| visibility |
unrestricted-access |
| dateCreated |
2020-01-30T08:19:26+09:00 |
| dateModified |
2021-06-24T13:10:22+09:00 |
| datePublished |
2021-06-24T13:10:22+09:00 |