| treatment |
A lysate was prepared from evacuated BY-2 cells. We incubated AGO7 mRNAs, TAS3 mRNAs and SDE5 mRNAs in the lysate. |
| growth_protocol |
BY-2 cells were cultured in 100 ml the medium (KOH, pH 5.8) containing 0.46 g Murashige and Skoog Plant Salt Mixture (Wako), 3 g sucrose, 0.1 mg Thiamine hydrochloride, 10 mg myo-Inositol, 20 mg KH2PO4 and 0.2 g 2, 4-Dichlorophenoxyacetic Acid. |
| cell_line |
BY-2 cells |
| description |
AGO7 treated small RNAs from in vitro recapitulation system (tobacoo cell free system) |
| growth_condition |
incubated at 130 rpm by shaking in a dark at 27 degree celsius |
| sample_title |
AGO7-only_small RNAs from in vitro recapitulate system |
| sample_type |
cell culture |
| sample_name |
S2_AGO7-only_small RNAs from in vitro recapitulation system |